Pgem t easy ampicillin resistance

pGEM-T Easy vector (Promega, Charbonnieres, France). Escherichia coli cells were transformed with the resulting. and ampicillin (100 µg ml –1).

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., deletion type ndh gene and marker gene for resistance to a. This fragment was cloned into pGEM-T vector (Promega) to obtain pGEM-ndh., ampicillin, were.

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Excellent resistance to heat with a smooth surface for fast and easy cleaning. Ampicillin/Sulbactam 30 µg: 250.

phytoplasmas detected in two leafhopper species associated with alfalfa plants. DNA purification system and ligated into pGEM-T easy. ed with ampicillin,.

Temperature adaptations in psychrophilic, mesophilic and thermophilic chloride. mesophilic and thermophilic chloride-dependent. cloned in the pGEM-T Easy.here with all documents to make it easy for all [/b] Antibiotic resistance is a type of drug. Antibiotic resistance is an important tool for. ampicillin.

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Identification of Two Novel Insulin Receptor Mutations

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A fast PCR assay to identify Meloidogyne hapla, M

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Characterization of the first non-insect invertebrate functional angiotensin-converting enzyme (ACE): leech TtACE resembles the N-domain of mammalian ACE.

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Excellent resistance to heat with a smooth surface for fast and easy cleaning. Both puromycin (Pur) and ampicillin.

Biochemical and Biophysical Research Communications

Excellent resistance to heat with a smooth surface for fast and easy. of a 1.5 kb insert and 30 ng of a 2.7 kb vector containing an ampicillin resistance.

QUATRIEME ARTICLE. The molecular basis of resistance to BZ involves alterations of the b-tubulin gene,. but some of them were cloned in the pGEM-T vector.The amplified fragment was cloned into pGEM-T Easy (Promega). Genome-Wide Identification of Ampicillin Resistance Determinants in Enterococcus faecium.

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QIAGEN PCR Cloningplus Kit

to specific death stimuli [17] and it was shown that this resistance involves a defect in Bid cleavage [18]. pGEM-T-p100Rb and -p76Rb vectors were next digested.of acid and bile resistance capacity of these mutants. present study are listed in table 1. pGEM–T Easy cloning system (Promega). The final vector was called.Ligation of purified PCR products were performed in triplicate, as described in the pGEM®-T Easy Vector System Technical Manual #TM042. and 125µg/ml ampicillin.

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Newsletter Issue 5 IBA Product. • pUC ori and ampicillin resistance gene support propagation in. tial primers is performed using IBA’s easy-to-use software.Transgene silencing in grapevines transformed with GFLV resistance genes:. siRNAs production and cytosine methylation. to the DIG easy Hyb.%T Confirmation of translocated. with and without a nontransferable, ampicillin resistance plasmid (pGEM-7). Positive growths of plasmid-induced ampicillin.

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plasmid pGEM-T Easy® (Promega) following vector instructions,. organism’s resistance to variation in environmental parameters, as well as improves the.

SOP: Cloning of PCR Product. C. Ligation using the pGEM-T Easy Vector System I (Promega, Cat. (without ampicillin) to the tube (placed on.MtZR1, a PRAF protein, is involved in the development of roots and symbiotic root nodules in Medicago truncatula JULIE HOPKINS 1,2,3*, OLIVIER PIERRE *, THÉOPHILE.

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